6/30/2023 0 Comments Shelf life of klenow fragment![]() Assignors: ARMES, NIALL A., PIEPENBURG, OLAF, STEMPLE, DEREK L., WILLIAMS, COLIN H. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Priority claimed from US35856302P external-priority Priority claimed from US10/371,641 external-priority patent/US7270981B2/en Application filed by Alere San Diego Inc filed Critical Alere San Diego Inc Priority to US12/802,862 priority Critical patent/US8017339B2/en Assigned to ASM SCIENTIFIC, INC. Original Assignee Alere San Diego Inc Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) Stemple Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Granted Application number US12/802,862 Other versions US8017339B2 Google Patents US20110059506A1 - Recombinase polymerase amplification US20110059506A1 - Recombinase polymerase amplification ![]()
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6/29/2023 0 Comments Cyberduck s3 http profile![]() ![]() ![]() # IF package versions are older than 0.4.4-6 then # install yum-plugin-merge-conf package and # update existing nodefabric-* rpms Yum list installed | grep nodefabric-core # check existing nodefabric package versions ![]() If you are running NodeFabric version older than 0.4.4 you need to update nodefabric-core-* rpms and to pull required service containers. Update NodeFabric to latest (DEV) version Their inclusion into pre-built NodeFabric images is planned for upcoming NodeFabric v0.4.4 release - so for now you can pull them through a simple nodefabric-update procedure. NB! At the time of writing this article nf-ceph-rgw and nf-rabbitmq containers were not yet part of latest NodeFabric Host Images release (as of v.0.4.3). However it does not need special bootstrap procedure - it is enough to simply enable nf-rabbitmq docker instances across NodeFabric cluster nodes. It is packaged as nf-rabbitmq service container and currently it does require 3-node NodeFabric cluster to run on - as it is fully clustered service and provides highly-available message queues. RabbitMQ is an open source message broker software that implements the Advanced Message Queuing Protocol (AMQP). Yet its internal load-balanced service endpoint is auto-created and bound to local docker0 interface as usual. It requires that you have Ceph Monitors and OSDs already running - for detailed instructions how to bootstrap Ceph in NodeFabric please refer to this user-guide section.Ĭeph Object Gateway is packaged as nf-ceph-rgw service container and it is basicly "standalone" - so nf-ceph-rgw docker instances do not need any special cluster bootstrap procedure - just enable them on one or more nodes. AboutĪmazon S3 and Swift object storage APIs can enabled by running Ceph Object Gateway (ie radosgw) service on top of existing NodeFabric Ceph storage cluster. In this article we are looking how to implement Amazon S3 and Openstack Swift object storage based on Ceph Object Gateway (radosgw) service and how to run highly-available RabbitMQ messaging cluster - all turn-key with NodeFabric. aws s3 cp /data/postgresql/dropbox/2 s3://healthcareblocks-storage-12345 -sse Downloading a file aws s3 cp s3://healthcareblocks-storage-12345/stuff.txt myfile.21 November 2015 howto, nodefabric Deploying private AWS S3/Swift and RabbitMQ Listing Bucket ContentsĪssuming your bucket is called healthcareblocks-storage-12345: aws s3 ls s3://healthcareblocks-storage-12345 Uploading a local file aws s3 cp myfile.txt s3://healthcareblocks-123Īlso, note that you don't need to be in the same directory as the file, since the aws command works from any directory, e.g. S3 does not encrypt or obfuscate object names, therefore it is important that you do not embed patient names, SSN, and other PHI identifiers in the file names of the objects saved to S3. Healthcare Blocks enables encryption-at-rest for newly created S3 buckets. The AWS SDK (for each programming language) contains pre-signed S3 URL functionality - please refer to the respective documentation. This prevents users from distributing a valid object URL externally. If you need end users to be able to retrieve content directly from S3 (even if your application obfuscates the S3 object URL and does an internal redirect), your application should create a pre-signed token that automatically expires within 5 minutes. Application-Level Authenticationįiles containing PHI should have their S3 object permissions set to "private" via the AWS SDK. If you require access to your bucket outside of the Healthcare Blocks environment, please create a support ticket, and we'll allocate a key pair you can use externally. Thus, any command issued through the AWS command line interface or through one of their SDK's will work automatically without the need to add a key pair to your environment. Your server is associated with a custom profile that grants it full permission to your Amazon S3 bucket. ![]() If you have been granted access to an Amazon S3 bucket for storing data, here's how to interact with the contents: Authentication ![]() 6/29/2023 0 Comments First class trouble on xbox![]() Cooperation is key to success, but trust will be in short supply as players attempt to figure out who the robots in their ranks are. What began as a relaxing vacation to outer space has erupted into a full-blown A.I. ![]() The ship is full of robot servants that are more focused on serving pain than beverages and some of the survivors are robots in disguise. The band of surviving players must now work together to try and reset the AI to revert it to a non-homicidal state, but it’s not going to be easy. They wiped out most of the passengers by simply turning the air off, but a lucky few with Oxygen Rebreathers survived. Chief of these problems is that the AI and on-board robotic servants have rebelled and decided to kill every human onboard. After all, in space, no one can hear you - Beep Beep, Boop Boop - I. Interested players can also sign up to take part in the game’s Alpha, which will take place on May 10th. In First Class Trouble you take on the role of one of the last remaining survivors on a luxury spaceship that has experienced some severe technical difficulties. First Class Trouble is expected to be available for Nintendo Switch, PlayStation 4, and Xbox One in 2020, with Early Access coming to Steam in Q4 2020. Early Access (PC) Available Now First Class Trouble is a social deduction party game set on a luxury galactic cruise ship, where players must work together. Marvel’s Midnight Suns for PS4 and Xbox One launches May 11 alongside DLC. ![]() ![]() Summary: First Class Trouble is a party game where players must work together and against each other to survive a disaster. It was released on Steam for Microsoft Windows under early access on April 8, 2021, before having its 1.0 version being officially released on November 2, 2021. First Class Trouble is a social deduction survival game developed by Invisible Walls and published by Versus Evil. Generally unfavorable reviews based on 5 Ratings. First Class Trouble is a party social-deduction game developed by Invisible Walls and published by Versus Evil. ![]() 6/29/2023 0 Comments Rust for beginners console![]() Software features a competitive multiplayer mode, in which players can battle each other for resources. Multiplayer-only game, as such, players must interact with each other in order to survive. Program is challenging and rewarding, player can spend hours trying to survive. ![]() Player can build structures to protect themselves from elements and other players. Player starts with nothing, must scavenge for resources to craft items. Player must be careful not to attract too much attention from game’s aggressive wildlife. You may find during play Rust online that it’s challenging, requires strategic thinking. Player must gather resources, build shelter and craft weapons in order to survive. Program is very challenging, players will need to use all of their survival skills to stay alive. There is no set goal, players are free to do whatever they want. Program Rust on Mac does not have any loading screens, transitions between areas are seamless. Graphics are realistic, environment is detailed. Environment is well-rendered, character models are realistic. The graphics are one of game's strongest points. Software runs smoothly and there are no major graphical glitches. Environment is dark and gritty, characters are well-rendered. Graphics in Rust unblocked are very realistic and detailed. ![]() Program is set in a post-apocalyptic world where players must scavenge for resources to survive. If we talk about Rust for Android, it’s a multiplayer-only game that pits players against each other in a fight for survival. Product has no set goal, players can choose to either cooperate or compete with each other. Software was released in Early Access on December 11, 2013, and became available on February 8, 2018. ![]() Rust is a first-person shooter video game developed and published by Facepunch Studios. ![]() 6/29/2023 0 Comments Billie bossa nova album![]() ![]() In a January 2020 interview, Eilish stated she would begin working on her upcoming second studio album during the year. Eilish released a number of singles since the album: the stand-alone " Everything I Wanted" (2019), the James Bond theme song " No Time to Die" (2020), and the Rosalía collaboration " Lo Vas a Olvidar" (2021), the first two of which won the Record of the Year and Best Song Written for Visual Media at the 63rd Grammy Awards, respectively. The album and the single helped Eilish win five awards at the 62nd Annual Grammy Awards, including the "big four" Album of the Year, Song of the Year, Record of the Year, and Best New Artist. ![]() It was met with critical acclaim and commercial success, and spawned the Billboard Hot 100-topping single " Bad Guy", her first number-one on the chart. To further promote the album, Eilish embarked on her sixth concert tour, titled Happier Than Ever, The World Tour, which began on February 3, 2022.Įilish released her debut album When We All Fall Asleep, Where Do We Go? (which was also produced by her brother) on March 29, 2019. Eilish performed the album's tracks in the Disney+ concert film, Happier Than Ever: A Love Letter to Los Angeles, which was released on September 3, 2021. Happier Than Ever debuted atop the Billboard 200 as Eilish's second number-one album in the United States, and topping the album charts in 27 other countries. "Therefore I Am" was the highest-charting song from the album, peaking at number two, followed by "My Future" at number six, and "Your Power" at number 10. Seven singles were released in promotion of the album: " My Future", " Therefore I Am", " Your Power", " Lost Cause", " NDA", " the title track", and " Male Fantasy" the first three peaked within the top 10 of the US Billboard Hot 100. At the 64th Annual Grammy Awards, the album and its title track received a total of seven nominations, including Album of the Year, Best Pop Vocal Album, Song of the Year and Record of the Year. Upon release, the album received acclaim from music critics, who praised its stylistic, restrained production, and insightful lyrics. Consisting of torch songs about the downsides of stardom, Happier Than Ever draws heavily from Eilish's rise to fame and the drawbacks that come with it. Primarily a downtempo pop record, Happier Than Ever is characterized by sparse, jazz-influenced, electropop arrangements set to meditative tempos, departing from the upbeat, trap-led sound of Eilish's debut album, When We All Fall Asleep, Where Do We Go? (2019). Eilish cited self-reflection during the COVID-19 pandemic as the biggest inspiration for the record. Eilish co-wrote the album with her brother and frequent collaborator Finneas O'Connell, who also produced the album and played every instrument. I'm not sentimental Comment by S_Unicorn51Īnd look at me a little more Comment by marce.Happier Than Ever is the second studio album by American singer Billie Eilish, released by Darkroom and Interscope Records on July 30, 2021. :( why would she put it on go Comment by S_Unicorn51 :( why would she put it on go Comment by dia✨ I have no shame in saying it's better than mine. ![]() SUCKIN DICK EATING SHYT AND SNITCHIN Comment by Abrahanmovicīaby I'm not sentimental. ]who tryna collab Comment by BACK TO THE GLITCH This was sincerely a great song Comment by departing Good day to this community! I just released two singles which blend elements of cloud rap, dream pop and psychedelic pop and I believe this esteemed community would be interested in checking it out. Thanks alot, brothers and sisters! Comment by Son Of Whyachi I would really appreciate listening and giving your review and if you enjoy the music, like and follow. 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This Wii entry on the popular series features a number of new additions, including an Adventure Mode with co-op play, with new characters and stages, and online combat for multiple players with face off against each other from anywhere!. Download Super Smash Bros Melee (U)(OneUp) ROM / ISO for GameCube from Rom Hustler. ![]() Melee (E) ROM now and enjoy playing this game on your computer or phone. This is the Europe version of the game and can be played using any of the GameCube emulators available on our website. Super Smash Bros Melee Iso Zip - Jody#39s Site.ĭescription Have fun playing the amazing Super Smash Bros. Melee (v1.01) ROM now and enjoy playing this game on your computer or phone. This is the USA version of the game and can be played using any of the GameCube emulators available on our website. Melee (v1.01) game for Nintendo GameCube. Have fun playing the amazing Super Smash Bros. Download Super Smash Bros Brawl ROM for Nintendo Wii Roms and Play Super Smash Bros Brawl Video Game on your PC, Mac, Android, or iOS devices!.ĭescription. ![]() Super smash bros psp free download - SourceForge. M Super Smash B The Legend of Zelda A Link to the P The Legend of Zelda The Minish C WarioWare I. Super Mario Galaxy (USA) (En,Fr,Es) Super Mario RPG Legend of the Seven S Super Mario S Super M Super Smash Bros. Melee (USA) Topics game Collection cdromimages vintagesoftware Language English A game where famous Nintendo characters scrap each other up. This item does not appear to have any files that can be experienced on A Please download files in this item to interact with them on your computer. GitHub - UnclePunch/Training-Mode- A modpack for Super Smash Bros. Melee file size - 1.0GB is absolutely safe because was tested by most trusted antiviruses. Melee is a GameCube emulator game that you can download to your computer and enjoy it by yourself or with your friends.Super Smash Bros. ![]() Melee ISO is available in the USA version on this website. As for the differences between them i don’t really know, some later versions have fixes maybe an expert can clarify in a comment? The Dolphin Emulator Wiki needs your help!. Offering all versions, you only need one. Melee is a Action/Fighting video game published by Nintendo released on December 2nd, 2001 for the Nintendo Gamecube. Melee ISO Download in Description (Updated 2021) 137,233 views 1K Dislike Share Save. Melee ISO Download in Description (Updated 2021) - YouTube Super Smash Bros. Super Smash Brothers Melee ISO lt GCN ISOs | Emuparadise. Features include 4-player split-screen action, enemy AI to play missing players, 15 ships, 9 levels and plenty of fun blowing up the level and the other players to smithereens! 1 Review. A 2D game inspired by Gravity Power, Star Control and Super Smash Bros. ![]() Fue lanzado en Japón y América del Norte en 2001, y en Europa y Australia en 2002. Es el segundo juego de la serie Super Smash Bros., luego del lanzamiento en 1999 del juego original. Melee es un juego de lucha crossover desarrollado por HAL Laboratory y publicado por Nintendo para la consola de videojuegos GameCube. Super Smash Bros Ultimate Pc Download - MAC DOWNLOAD powered by Doodlekit. > DOWNLOAD: Smash bros melee iso download Smash bros melee iso download JSmash bros melee iso download ![]() ![]() ![]() ![]() Ultimately I would like to have all of this data placed into a. Here’s some common XPATH commands.I am trying to build a scrapy bot capable of ripping the data from my local craigslist for jobs as well as having a recursive functionality to allow for the contact data to be gathered as well. By inspecting the webpage, you can also look into which pieces of code you want to loop through, isolate, and extract. Initially try different xpath commands until you can isolate pieces of HTML that you want to grab. It should bring you to the shell window where you can test parsing the current website that you linked to. Now the easiest way to customize these for personal projects is to do lots of testing on the ScraPy shell. So how do we actually grab the information from the webpage? HTML and XML extracters. The parse method gets called after the Spider gets initialized and crawls the first url. #loop through the postings for i in range( 0, len(postings) - 1): Now what we need to do is parse through each hundred of the craigslist postings. The Spider automatically goes through the list of start urls. To follow the next page, instead of finding the “Next” button using a parser, we can kind of cheat the system by iterating over the different URLs by constructing them by scratch. If you test it out on craigslist, you’ll see that they have the base url for the first page, then “?s=100” for the next hundred, and then “?s=200” for the next hundred after that. Because every website usually has a syntax string format for subsequent url pages, it makes traversing through and grabbing the next page rather easy. We have to initially set up a base url that the spider will follow and loop through. #Initially grab all of the urls up to where craigslist allows #In this case, it's 2400 for i in range( 1, 24): Here I’ve created a constructor to initialize the fields that I want. One Item will generate exactly one row in the data-set, and then will continually loop. So when Scrapy crawls through each posting, it will generate an Item class and populate these values with what we tell it to grab. ![]() Since our example is Craigslist postings, I want to initially retrieve a lot of information about each posting such as the date, title, etc. ![]() Here I’ve done the non-traditional approach of just including it in my spider which is perfectly okay, but most people create a separate file for it.Īt the most basic form, each attribute or piece of information you want to store gets put into an Item. If you want to export your data at all, you’ll need an ITEM class. These are essentially a form of Python dictionaries that have been adapted into ScraPy for storing information. WordPress is glitching out and refusing to let me link anything. Here’s a quick and practical tutorial.įor the full code visit. Spotify has Spotipy…etc… This stuff is exciting. Lame! Discovering Scrapy had to be the first revelation to the awesome array of different cool packages Python has to make every single web application on the internet easy to use. I asked her if she used sci-kit learn for analysis and she said that she used Python. It was pretty cool except for one thing, they were all the same dataset! Predicting salary based on job descriptions. I asked a student about where she got her dataset and she said she found it on the internet. Last quarter I was hanging around the CSE atrium when different people from a joint statistics and cse machine learning learning class were presenting their final projects. But overall, this is where I think scraping actually establishes creativity. I mean if you’re using my scraper, you might be gaining insights into a different city. You’re doing something that has never been done before. No longer are you restricted to online directories of datasets that everyone has been accessing forever.ģ. It expands your breadth of possibilities. Perfectly formatted datasets never get presented to you.Ģ. I also think everyone who is into data science should definitely learn scraping for a couple of reasons.ġ. I thought the documentation was initially a little confusing. I wanted to introduce some practical methods of scraping using ScraPy as well as create a README for the Craiglist scraper code. ![]() |